Though improved fitness may prove true in the Jingwei et al. Harmful phenotypes brought about by chimeric proteins After further studies accomplished by Long M et al., it was concluded that this amalgamation of different protein domains resulted in a more favored selection. This new-fangled gene acts on long-chain alcohols and diols, such as pheromones and hormones, resulting in a direct change in the Drosophila’s fitness (ability to foster viable fertile offspring). The chimeric protein that resulted from these genes was derived from a gene that codes for alcohol dehydrogenase, and the yellow emperor gene. In contrast, some chimeric genes may result from errors in DNA replication or repair.įor example, one peptide chain might have varying properties which could contribute to binding, recognition, or even toxicity, while its fused counterpart could aid in the targeting and stabilizing of the chimeric polypeptide.Īn example of a chimeric gene, brought about by the joining of two or more genes that initially coded for separate proteins, are the Jingwei genes found in Drosophila. One is artificial, through two or more predetermined coding sequences. These chimeric genes can originate in two different ways. These fabricated chimeric genes often take the form of a plasmid or virus, an effective hull for gene manipulation. The genes will then be transcribed from the DNA, resulting in mature mRNA that will then be translated into a cohesive polypeptide chain, forming our chimeric protein in question. To code for chimeric proteins, one must first construct the chimeric genes. Creating chimeric genes and chimeric proteins In creating these expression vectors the target of regulation is often transcription. With this genetic manipulation to create chimeric proteins, we can control the activity of many specified proteins, providing new insights into the field of therapeutics. Site-directed mutagenesis performed with certain expression vectors can allow for these gene fusions. Translation of these chimeric genes generates a single polypeptide chain, expressing different functionality from the original proteins from which it was derived. Simply put, these chimeric proteins are a result of the fusion of chimeric genes through structural machinery (RNAs, rRNAs, tRNAs). By Vasco Medeiros, BSc Reviewed by Emily Henderson, B.Sc.Ĭhimeric proteins are fashioned through in vitro methodologies.
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